伏马菌素B1单克隆抗体的制备及免疫学检测方法初步应用

doi:10.11843/j.issn.0366-6964.2016.05.019

Abstract

Abstract:

A monoclonal antibody against fumonisin B₁(FB₁) was developed and applied to establish rapid immunology detection method of FB₁.FB₁was successfully conjugated with BSA and OVA using EDC，respectively.Hybridoma cell line secreting monoclona antibodys (mAbs) against FB₁was produced by fusing murine SP2/0 cells with the spleen B cells of BALB/c mice which was immunized with FB₁-BSA for four times，every four weeks at a time.The mAbs against FB₁were obtained by inducing ascites in the mice body.The affinity，titer，substype and sensibility of the mAbs were assayed with ELISA.Furthermore，the mAbs were preliminarily applied in immunology detection of FB₁.The mAbs against FB₁ whose substype was tested as IgG₃ were secreted by hybridoma cell line (2E11-H3).The working concentration of the mAbs was 1∶6.0×10⁴，affinity constant was 7.98× 10¹⁰ L•mol^-1，IC₅₀was 43.65 ng•mL^-1.The cross reaction ratios with Fumonisin B₂ and B₃ were accordingly 385% and 72.4%，while none with other analogues and carrier protein.The recovery rate of sample was among 85.85%-114.07%，the average was 98.81% and the coefficient of variation was 10.11%.The monoclonal antibody against FB₁ with high affinity，specificity and sensibility was produced and preliminarily applied in immunology determination of FB₁.

CLC Number:

S859.8

YAO Jing-jing，HU Xiao-fei，HAN Jun-ling，LI Qing-mei，WANG Fang-yu，DENG Rui-guang，ZHANG Gai-ping. Development and Preliminary Application of the Monoclonal Antibody against Fumonisin B₁[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1009-1017.

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References

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Development and Preliminary Application of the Monoclonal Antibody against Fumonisin B₁

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Development and Preliminary Application of the Monoclonal Antibody against Fumonisin B1

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Development and Preliminary Application of the Monoclonal Antibody against Fumonisin B₁